FlexIII. These models can no longer be repaired. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. A polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. Highly selective for dsDNA over ssDNA or RNA. High tolerance for contaminants. Compatible with Molecular Devices SPECTRAmax 190 and 250 plate readers. softmax pro software, gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell. Our. p-Nitroaniline - Catalog No. Both reactions can be easily measured in Molecular Devices’ new SpectraMax® L microplate luminometer. Plate and Setup Rack Options Screens with selection of up to four Methods shown. validation, maintenance, and repair documentation. Visit the supplier page or submit a quote to learn more. . Site. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. x. 0 . Janet got excellent results using them and they are only ~$1. Absorbance cuvette port. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. d. The StakMax stacker also allows users to feed plates into the loading area manually. 96-well PCR plates are plastic, heat-resistant multi-well microplates designed for use inside PCR machines or real-time (qPCR) thermocyclers. White plates reflect the light, enabling even low levels of Luminescence to be detected. Flexible liquid transfer Using an 8- or 16-channel pipettor, the FlexStation 3 Reader offers added assay flexibility over dispenser-based systems by transferring reagents from 96 or 384 distinct wells in a source plate to a read plate, simultaneously. 10 µL of BSA standard or cell lysate was pipetted into wells of a 96-well microplate (n = 3). MCF7 cells treated with. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. The 8-channel read head allows for fast read times which means a 96-well plate can be read in as little as five seconds. Absorbance Plate Reader Comparison Chart. A standard curve consisting of MCF7 cells seeded from 20 to 20,000 per well in a 96-well plate was linear across the entire range with r 2 = 0. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well microplates. Two high-energy xenon flash lamps: Reading capabilities. x. 3) At the bottom of the strip, click on "Show. Microplate Readers include the M3, M4, M5, and M5e models. Page 1 of 8 . Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. Answer. Make sure toPlate reader instructions Computer. A control well was set up to verify. Products : Ionworks HT, Ionworks Quattro. Instrument settings can. Now select the area of the plate to read. Anticancer drug assay was also conducted using conventional 96-well plates as controls. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. The eight-channel system delivers high precision and speed when reading 96-well microplates for single endpoint measurements and kinetic assay data gathered over time. To test the optical clarity of the various microplates at low wavelengths, one well of each plate was filled with 100 μL (96-well) or 20 μL (384-well) distilled water, and its. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the assay. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 150 µL of deionized water was pipetted into each well of a clean 96-well plate. Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The Versamax Microplate Reader is built around a grating monochromator. Gemini EM Reader scans. One plate received 200 µL of the BCA working reagent, and the other received 200 µL of the Rapid Gold BCA working reagent. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. It has a 340-750nm Spectral Range. #3603) SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. UV absorbance detection. UV Plate 96 Well, Flat Bottom, Cs 50. Published Dec 16, 2020 | Updated Jun 22, 2023. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. #C1500) Control standard endotoxin (ACC cat. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. 1. Get Quote . Endpoint, kinetic, spectrum. Molecular Devices delivers a robust screening platform to address real-time kinetic cell-based assays with the FLIPR Tetra® System. 00. 4 (Molecular Devices cat. The SpectraMax Quant dsDNA Assay Kits provide pre-optimized, complete solutions that are sensitive, accurate, and easy to use. to each well. #6590-0137) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax iD5 reader are shown below. T = I/I 0 and %T = 100 (T). Used. (Molecular Devices cat. 0 OD. Create custom fluorescence assays without having to purchase expensive filter sets. An entire 96-well plate can be processed in less than six seconds. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. Artel MVS Bundle DM Std/800TSNB Reader Details. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. 0 fmol/well FITC 200 µL in 96 wells Bottom-read detection limit. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. Description. The system features 96, 384,. It offers extremely low absorbance background, excellent well to well uniformity, and zero percent cross talk. Spectrofluorometer from Molecular Devices® provides a flexible environment. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. C. Fluorescence Microplate Reader Comparison Chart. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. Two different agonists, acetylcholine and carbachol, were tested. The SpectraMax ABS readers can be integrated with Molecular Devices StakMax® Microplate Stacker within . The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. x. Cayman Chemical. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. These instruments deliver high precision and speed when reading 96 Well Microplates for single endpoint measurements and kinetic assay data gathered over time. with Quartz ABS Plastic. of Dispensers:Molecular Devices deliver high throughput while showing good correlation with manual. UV cuvettes are ideally suited for measurements at 260 nm, 280 nm and in the visible range. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Figure 2-23: Bottle holder To install the bottle holder: 1. Place the microplate in the drawer. From the dropdown Menu, select Wellplates. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. flat-bottom: 50-60 µl. The instrument reads any standard cuvette, microcuvette, test tube and 96 well or UV transparent microplate over full 190-1000 nm wavelength range. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 2. 0% ± 0. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. Our washers provide speed and configurability options—from single-tube dispensing to washing full 96- and 384-well plates. 90349). $3,207. sdale innersh Triangle Woingham ershire RG41 T United Kingdom 11 umolde. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. A 1:10 dilution series of ATP was set up in solid white 96- and 384-well plates at concentrations from 10 uM down to 1 nM, along with blank wells containing no cells. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. The absorbance was then read on the SpectraMax microplate reader at 426 nm, and the resulting OD values for all wells were averaged together to obtain a value of 0. 00. Instrument settings can beCorning 96-well black microplates with clear bottom (Corning cat. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. Elevated well walls for easier sealing Round bottom formats Square and round well formats Conform to SBS standards Lids and cap mats available Packed in sleeves of 6, 24. These are the default kind of cuvette & 96 well plate stocked by the lidstrom lab as of 11/2013. In SoftMax Pro, Dual Read mode was selected. Three kits covering a wide range of dsDNA concentrations. 0 . 000 ABS • Resolution: 0. 96-well Molecular Devices P/N 42-000-0117 HE™ High Efficiency microplates, 384-well Molecular Devices P/N 0200-5201. Cell viability and cytoxicity assays. • High antibody-binding surface improves the overall performance in. 384-WELL SBS STERILE MICROPLATE. Kinetic assays were read every 20-28 seconds for one hour,The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. 300 µL of Detergent Compatible Bradford reagent was added to each well. Reader Documentation Version No. Compare this item. • Remove media from each well using a blue pipette tip being careful as not to remove cells. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. $488. The Phosentix MicroQC™ Plate is an advanced microplate reader analysis tool that offers exceptional multi-modal functionality. 2. Important things to consider when purchasing a multimode microplate reader. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). On the day of the assay, the plate was equilibrated. We would like to show you a description here but the site won’t allow us. Wavelength(s):. 96/384-well plate formats. Morphology and fluorescence gates were set to select the best 96 clones secreting IgG antibodies. Plate Readers, Fluorometers & Luminometers; Lab Automation; Molecular Diagnostics. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. PURPOSE. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Your applications, your modes, your choice. 305 reference level saturation. This reader also makes use of a unique temperature-independent methodology for path length correction. Luminometers and fluorimeters allow for readout of luminescence (luciferase readouts) or fluorescence from an entire well of a micro-well plate such as a 96- or 384-well plate. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. A microplate adapter is required in the reading chamber drawer for 96- and 384-well assay plates. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and Bottom read. Evaporation protection (humidity cassette) Te-Cool™ (active temperature regulation 18-42 °C) Integrated lid handling. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Mode Plate Reader. The soil was amended with either 1. The 6-well plates were incubated at 37ºC for 9 days before picking. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. We would like to show you a description here but the site won’t allow us. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. Explore the best practices for running absorbance-based assays how Molecular Devices absorbance readers can be utilized to optimize said absorbance-based assays. Clear plates will give more cross talk between wells. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. )Plate dimensions: X6001, X6004, X6011, X7001, X7007, X7020, X7022, X5101, X6026. It offers extremely low absorbance background, excellent. x. Capabilities. Click Add to create the plate. Partner with our team of scientists and engineers who can customize your instruments, as well as automate entire workflows to meet the specific needs of your assay, method, or protocol. Therefore you need to ask a physicist. #ABS Plus) Cell plating and treatment prior to assay. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. The BCA assay plate was read at 562 nm, the original wavelength setting in the protocol, while for the BCA Rapid. 0200-6263. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. x. maximum-illumination-channels. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The plate was preincubated in the reader at 37°C for a minimum of 10 minutes. For the kinetic and endpoint LAL assays, the ELx808 TM Reader is highly reliable and easy to use. Cell viability and cytoxicity assays. 0. To assess the sensitivity of each microplate reader, a plate was set up with 32 wells containing PBS only. 312 gain calibration failed. Products : SpectraTest Validation Plates ABS2, SpectraTest Validation Plates ABS1, SpectraTest Validation Plates FL1, SpectraTest Validation Plates LM1. Figure 3. Each well of a 96- well microtiter plate is coated with a fluorescently labeled IgG-binding peptide, Protein G. SUPPORTED PLATES Microplates having 6, 12, 24, 48, 96, and. Fluorescence, Absorbance Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec Temperature Control: Ambient +5C up to 42C Shaking: Linear and Orbital. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. The. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the. Washers configured for both 96- and 384-well plates. Align the two slots on the bottle holder with the two pegs on the rail. 0% Normal and Rapid modes, 96- and 384-well plates; Absorbance Repeatability: 0. 1. x. NanoQuant plate. Contents of wells were pipetted up and down several times to mix. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Max, FlexStation, Gemini, and earlier instruments as well as import and analysis of data files from FLIPR and Analyst instruments. (That difference comes at a cost, Amouretti explains, as higher plate densities require better optics and more sensitive detectors. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. 314 low lamp level warning. 046 to 0. #CA961) Methods. 25% and 0. wells across each plate • Enables viewing and tracking of every well in every plate • Reveals additional information on cellular. 3 OD at 200 nm. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among others. and glow luminescence modes in 96- to 384-well plates. Products : General, FilterMax F5 reader. This approach enables uniform light dose application to 9 wells (a 3-by-3 matrix in a 96-well plate) simultaneously, which can be leveraged to shorten the duration of PDT experiments (Fig. Learn how you can utilize microplate readers to drive landmark discoveries. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. 5 µL of each sample was pipetted into a 96-well plate for PCR. Compact design Minimum volume and maximum throughput with 96- or 384-well microplate compatibility Powerful data analysis with SoftMax® Pro Software Optimal performance with validation tools Small but Mighty EarlyTox Live/Dead Assay Kit (Molecular Devices cat. The Fluorometric Imaging Plate Reader (FLIPR®; Molecular Devices, Sunnyvale, CA) has made a significant contribution to drug discovery programs in the pharmaceutical industry since the first commercial instruments were introduced 9 yr ago. x. • 96-well yellow plate 0200-6182 Pipettor head kit, 16-channel (384) for FlexStation 3 Reader • 16-channel pipettorElisa Plate Readers and Microplate Readers at Biocompare. #SpectraMax L Config) Methods. High-throughput, automated dynamic light scattering. Bioz Stars score: 86/100, based on 40 PubMed citations. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. Component A (2 vials*)Figure 1. 0 nm increments Dynamic range > 7 decades Cross-talk < 0. Selects either 96-well or 384-well plate for a stand-alone read. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. #E010) Pyroplate 96-well microplate (ACC cat. Microplates, cuvettes (via adapter) Detectors. Features. 384-WELL AND 96-WELL 10MM-HEIGHT STERILE MICROPLATES. 24-, 96-, 384-well plates and microwell strips; Manifold Channels: 96-well washing: 8-well (1x8) manifold, 2x8-well manifold, 12-well (1x12) manifold, 8-well short tube (1x8) manifold. 사용이 편리하고 견고한 생명과학 연구, 신약 개발, 개발, Bioassay용 assay. 9. Features: Standard back plate for use with hand sanitizer, hand lotion, or foam dispensers. We would like to show you a description here but the site won’t allow us. Molecular Devices SpectraMax Plus 384 Absorbance. No accessories are needed and SoftMax® Pro Software. 50/plate. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. This eliminates the need for standard curves, and 96–well: 3 mP 384–well: 3 mP 1536–well: 6 mP 1 LUMI Glow luminescence detection for different plates 0200-7014POS Glow for 96-well plates 96–well: 1 pM 96–well: 1. Absorbance values were read on the SpectraMax iD5 reader using a preconfigured BCA assay protocol (the SpectraMax iD3 reader is also suitable for these assays, as it has the same absorbance performance as the SpectraMax iD5 reader). For FlexStation I/II/3 plate readers, what are the dead-volumes for the compound plate? The FlexStation plate readers assume a 1 mm pipette height from the bottom of the well when aspirating from a compound source plate. 17,000 cells. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started. • Photometric range: 0. 5039228D 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 12 Applications 12 Optics 13 PlateControls 13The microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. x. Next generation SpectraMax® absorbance microplate readers. Wells of 96-well plates (NEST) were pre-coated with 100 μg/ml of collagen solution for 1 hour. Corning. An industry-renowned instrument for monitoring GPCRs and ion channels. Three low volume 24-well bottom slides. The DynaPro Plate Reader III provides unparalleled levels of productivity and flexibility for the most demanding nanoparticle sizing and protein aggregation tasks such as biotherapeutic formulation, screening of promiscuous inhibitors, and optimization of protein. Top and bottom reads are. 5 pM 1 0200-7015POS Glow for 96- and 384-well plates 96–well: 1. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. Multimode microplate reader equipped with latest detections like fluorescence,. Unparalleled performance on a personalized platform. minutes and begin reading microplates with seven mouse clicks. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. of 3 wells of a 96-well plate, from 1 of 3 independent experiments. . 96 Well Plate Reader found in: Well Plate Reader Accessory, SmartReader™ 96 Microplate Absorbance Reader, SmartReader UV-Vis Microplate Reader, Stratus. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Setting up the Instrument 1. Versa Max. Wells to read. Top or bottom detection is available with a simple click of a button. UV Plate 96 Well, Flat Bottom, Cs 50. 5°C at 37°C. The equation that allows one to calculate absorbance from % transmittance is. The SpectraMax Paradigm Platform was able to quantify the protein down to the low picomolar level using an AlphaLISA method (Figure 1). FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Materials. Instrument pricing. 4 decades . Quantitative, whole well cell confluence for every well. Published Dec 16, 2020 | Updated Dec 16, 2020. SpectraMax ID5 Plate Reader (Molecular Devices). SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. Molecular Devices. Description. This is equivalent to having 511 built-in filters, The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. Cross-talk <0. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Sample by eye within . ₹ 1,80,000 Get Latest Price. #23225). With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Product Compare 0. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, andthe iD5 reader’s filter system. A 96-well microplate reader for absorption spectroscopy was designed, constructed, and tested at a total cost of ca. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. To validate the assays’ ability to detect a wide range of viable cell numbers, a series of cell densities was set up. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. This can increase throughput and efficiency, thus saving time and space in the lab. • Gently wash cells once with 200 μl (for 96 well plate) or 400 μl (for a 24 well plate) warm (37°C) 1X DPBS (containing Mg and CaCl 2). Detection cartridge modularity allows for easy addition of new read modes within minutes without. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. 5 ng per well. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. Products : SpectraMax L reader How long does it take to dispense into a 96 well plate with SpectraMax L reader injection? For a 96-well plate it takes a total of 27. # SMP54-GXP- 10UL) Pyrochrome Assay chromogenic test reagent (ACC cat. EMax. Supplier: Molecular Devices. Running both standard spectrophotometer and microplate. Temperature control: 4°C above ambient to 45°C. 340 PC384. Wide range of applications The VersaMax reader covers a wide range of applications: Step 1: Turn on your microplate reader. µl) was added to each well and incubated at 37°C for 1 h. The VersaMax Microplate Reader is supplied with SoftMax® Pro Data Acquistion & Analysis Software, Molecular Devices' industry leading all-in-one data acquisition and analysis. Part of a plate can be selected so that only a selected area of the plate. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. Dimensions: 18. They are also sealable using different types of adhesive mats foils or films. Plate SpectraMaxiD3,iD5,i3x,i3,M2,M2e,M3,M4,M5,M5e,Plus. Bay Gray powder coated metal back plate, column, and base. No. 00 . 130009WMicroplate Readers. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader.